Creating populations with filters
Creating gated populations is one of the most important tools when evaluating your results data. There are multiple tools you can use to create gated populations. Simply click on the Create filter to see these tools.

Manual Gating

You can gate populations manually by using the Rectangle, Oval, or Freeform tools.
After selecting Rectangle tool, gate around desired population to create.
Once you selected the population, you need to name that population and it will automatically be saved under population tree.
GFP+ population created by rectangle tool.

Statistical Gating

The By Statistics gating tool allows you to gate populations based on standard deviation or threshold.
When you select By Statistics, a new window will appear where you can toggle between Standard Deviation and Simple Threshold gating.
Standard Deviation gating
For Standard Deviation gating, you can specify how many standard deviations (SD) from the mean you want to use. For the SD value, you need to specify the desired settings for how the SD value should be applied. Choose the less than (<) or greater than (>) sign and specify either above or below the mean.
Lastly, you need to define what metrics (channel, cell, nuclear size, etc.) to use for gating.
Simple Threshold gating
For Simple Threshold gating, you need to specify the threshold and decide if you want the population Below or Above the threshold. Lastly, you need to define what metrics (channel, cell, nuclear size, etc.) to use for gating.

By Image

This gating tool is used to select a specific image(s) you want to look at.
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